Hotspot

rps19-rpl2

IRb · 87722-87794 · 73 bp

Back to Eriobotrya

Pi 0.0314

MarkerSeek score 64.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 73
variable_sites: 7
pi: 0.0314
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCTCGGGACCAGGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	58.6	58.1	201-243 bp	1.0	81.2
rps19-rpl2_p2	GCTCGGGACCAGGTTACTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.6	58.1	293-335 bp	1.0	80.5
rps19-rpl2_p3	GCTCGGGACCAGGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	58.6	58.3	203-245 bp	1.0	79.8
rps19-rpl2_p4	GCTCGGGACCAGGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	58.6	57.8	259-301 bp	1.0	79.7
rps19-rpl2_p5	GCTCGGGACCAGGTTACTAT	TTTGATTCTTCGTCGCCGTA	55.0	45.0	58.6	57.6	202-244 bp	1.0	79.2