Hotspot

rpl33-rps18

LSC · 71004-71243 · 240 bp

Back to Eriobotrya

Pi 0.0331

MarkerSeek score 78.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 26
pi: 0.0331
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	381-437 bp	1.0	82.7
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	380-436 bp	1.0	80.3
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	382-438 bp	1.0	80.3
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	378-434 bp	1.0	79.7
rpl33-rps18_p5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	386-442 bp	1.0	79.3