Hotspot

psaJ-rpl33

LSC · 70340-70802 · 463 bp

Back to Eriobotrya

Pi 0.0115

MarkerSeek score 69.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 23
pi: 0.0115
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GTTCGGGTCTTTAGCAGGTT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	605-729 bp	1.0	76.8
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTT	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	632-756 bp	1.0	76.5
psaJ-rpl33_p3	TGGCACCGGTAGTAAGTACT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	630-754 bp	1.0	76.4
psaJ-rpl33_p4	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	560-684 bp	0.954545	74.9
psaJ-rpl33_p5	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	587-711 bp	0.954545	74.6