Hotspot

psaI-ycf4

LSC · 62642-63069 · 428 bp

Back to Eriobotrya

Pi 0.018

MarkerSeek score 67.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 20
pi: 0.018
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GTTTTTCGAGACCCCGCTAT	45.0	50.0	58.0	58.0	517-545 bp	1.0	86.7
psaI-ycf4_p2	GGGCTTAGTATTTCCGGCAA	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	526-554 bp	1.0	86.5
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.0	57.9	512-540 bp	1.0	86.4
psaI-ycf4_p4	TCAACAGTCTCCCCTCCATT	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	560-588 bp	1.0	86.3
psaI-ycf4_p5	ACTCTCAACAGTCTCCCCTC	GTTTTTCGAGACCCCGCTAT	55.0	50.0	58.1	58.0	564-592 bp	1.0	86.1