Hotspot

accD-psaI

LSC · 61716-62527 · 812 bp

Back to Eriobotrya

Pi 0.016

MarkerSeek score 75.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 59
pi: 0.016
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.4	58.0	855-957 bp	1.0	86.0
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	898-1000 bp	1.0	85.9
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	889-991 bp	1.0	85.8
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	50.0	55.0	58.4	58.1	851-953 bp	1.0	85.8
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	50.0	50.0	58.4	58.1	862-964 bp	1.0	85.7