Hotspot

rps4-trnT-UGU

LSC · 48483-48916 · 434 bp

Back to Eriobotrya

Pi 0.0225

MarkerSeek score 55.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 20
pi: 0.0225
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	540-594 bp	1.0	85.8
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	544-598 bp	1.0	85.5
rps4-trnT-UGU_p3	CTTCCGGCTCTAGGCTTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	532-586 bp	1.0	85.1
rps4-trnT-UGU_p4	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	508-562 bp	1.0	85.1
rps4-trnT-UGU_p5	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	512-566 bp	1.0	84.8