Hotspot

psbZ-trnG-GCC

LSC · 38404-38836 · 433 bp

Back to Eriobotrya

Pi 0.0407

MarkerSeek score 75.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 54
pi: 0.0407
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	469-697 bp	1.0	87.7
psbZ-trnG-GCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	455-683 bp	1.0	87.6
psbZ-trnG-GCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	425-653 bp	1.0	82.3
psbZ-trnG-GCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	456-684 bp	1.0	80.8
psbZ-trnG-GCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	470-698 bp	1.0	80.3