Hotspot

trnH-GUG-psbA

LSC · 145-397 · 253 bp

Back to Eriobotrya

Pi 0.0431

MarkerSeek score 68.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 32
pi: 0.0431
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	373-469 bp	1.0	89.7
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	ATTCCAAGCTGAGCACAACA	50.0	45.0	57.9	58.0	786-882 bp	1.0	86.7
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	TTCCAAGCTGAGCACAACAT	50.0	45.0	57.9	58.0	785-881 bp	1.0	86.7
trnH-GUG-psbA_p4	ATATTATGGGCGAACGACGG	CTGACGGTATGCCTCTAGGA	50.0	55.0	57.9	58.0	835-931 bp	1.0	86.4
trnH-GUG-psbA_p5	ATATTATGGGCGAACGACGG	TCTGACGGTATGCCTCTAGG	50.0	55.0	57.9	58.0	836-932 bp	1.0	86.4