Hotspot

rpl16

LSC · 81804-83332 · 1529 bp

Back to Erigeron

Pi 0.00724

MarkerSeek score 65.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1529
variable_sites: 53
pi: 0.00724
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAGAAACCTGCACCCACT	AGTTTCTTCTTTTCTGTTCAAT	45.0	27.3	57.8	52.1	1537-1725 bp	0.933333	43.4
rpl16_p2	AAAAGAAACCTGCACCCACT	TGGAACAAAAGAAATTAGTTTCT	45.0	26.1	57.8	52.7	1553-1741 bp	0.933333	43.4
rpl16_p3	AAAAGAAACCTGCACCCACT	TTAGTTTCTTCTTTTCTGTTCAA	45.0	26.1	57.8	52.5	1539-1727 bp	0.933333	43.4
rpl16_p4	AAAAAGAAACCTGCACCCAC	AGTTTCTTCTTTTCTGTTCAAT	45.0	27.3	57.0	52.1	1538-1726 bp	0.933333	43.4
rpl16_p5	AAAGAAACCTGCACCCACTA	AGTTTCTTCTTTTCTGTTCAAT	45.0	27.3	57.0	52.1	1536-1724 bp	0.933333	43.4