Hotspot

trnL-UAG-rpl32

SSC · 123630-124242 · 613 bp

Back to Erigeron

Pi 0.0069
MarkerSeek score 64.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
613
variable_sites
29
pi
0.0069
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGGAAATCTCTTTCTACCGGG 50.0 52.4 58.0 57.8 686-1011 bp 1.0 77.4
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGGAAATCTCTTTCTACCGGG 50.0 52.4 58.0 57.8 687-1012 bp 1.0 77.4
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC TGGGCATCGTTAAAAGCTTTG 50.0 42.9 58.0 57.7 713-1038 bp 1.0 76.6
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA TGGGCATCGTTAAAAGCTTTG 50.0 42.9 58.0 57.7 714-1039 bp 1.0 76.6
trnL-UAG-rpl32_p5 AGCGTGTCTACCGATTTCAC GGGCATCGTTAAAAGCTTTGT 50.0 42.9 58.0 57.7 712-1037 bp 1.0 76.6