Hotspot

rpl22-rps19

LSC · 84584-84719 · 136 bp

Back to Erigeron

Pi 0.051

MarkerSeek score 70.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 26
pi: 0.051
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAACTCTTCGCGCTTTGTGA	TCCGAGGACATGCAAAAAGC	45.0	50.0	57.9	59.1	195-271 bp	1.0	81.9
rpl22-rps19_p2	GAACGCCCACGAATTTGATC	TCCGAGGACATGCAAAAAGC	50.0	50.0	57.8	59.1	217-293 bp	1.0	81.7
rpl22-rps19_p3	TAACTCTTCGCGCTTTGTGA	TTCCGAGGACATGCAAAAAG	45.0	45.0	57.9	56.6	196-272 bp	1.0	79.9
rpl22-rps19_p4	GAACGCCCACGAATTTGATC	TTCCGAGGACATGCAAAAAG	50.0	45.0	57.8	56.6	218-294 bp	1.0	79.6
rpl22-rps19_p5	TAACTCTTCGCGCTTTGTGA	CCGAGGACATGCAAAAAGC	45.0	52.6	57.9	57.6	194-270 bp	1.0	79.6