Hotspot

ycf4-cemA

LSC · 60696-61672 · 977 bp

Back to Erigeron

Pi 0.0116

MarkerSeek score 71.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 62
pi: 0.0116
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	958-1181 bp	1.0	87.7
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	922-1145 bp	1.0	87.3
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	957-1180 bp	1.0	85.1
ycf4-cemA_p4	CTCCACGGGAAATGGAACAA	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.1	57.6	921-1144 bp	1.0	84.7
ycf4-cemA_p5	GGAGCCATTCCATTGACTCG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.7	58.1	959-1182 bp	1.0	82.3