Hotspot

trnG-UCC-psbD

LSC · 30913-32679 · 1767 bp

Back to Erigeron

Pi 0.012

MarkerSeek score 72.30

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1767
variable_sites: 104
pi: 0.012
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	ATGAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1480-1927 bp	1.0	80.8
trnG-UCC-psbD_p2	AATGAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1481-1928 bp	1.0	80.7
trnG-UCC-psbD_p3	GAATGAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	47.6	55.0	58.2	58.0	1482-1929 bp	1.0	78.9
trnG-UCC-psbD_p4	AATGAAACCGAAAGACCCCT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1499-1946 bp	0.933333	76.2
trnG-UCC-psbD_p5	ATGAAACCGAAAGACCCCTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1498-1945 bp	0.933333	76.2