Hotspot

ndhC-trnV-UAC

LSC · 50029-50986 · 958 bp

Back to Eriachne

Pi 0.0182
MarkerSeek score 69.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
958
variable_sites
84
pi
0.0182
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 947-1103 bp 1.0 59.1
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 948-1104 bp 1.0 57.2
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 946-1102 bp 1.0 55.1
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 948-1104 bp 1.0 55.1
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 947-1103 bp 1.0 55.1