Hotspot

petN-trnC-GCA

LSC · 17464-18373 · 910 bp

Back to Eriachne

Pi 0.0183
MarkerSeek score 70.10
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
910
variable_sites
92
pi
0.0183
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 913-981 bp 1.0 82.1
petN-trnC-GCA_p2 AGTCCACTTCTCCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 972-1040 bp 1.0 81.9
petN-trnC-GCA_p3 GTCCACTTCTCCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 971-1039 bp 1.0 81.9
petN-trnC-GCA_p4 AGTCCACTTCTCCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 974-1042 bp 1.0 81.4
petN-trnC-GCA_p5 GTCCACTTCTCCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 973-1041 bp 1.0 81.4