Hotspot

psbM-petN

LSC · 16593-17373 · 781 bp

Back to Eriachne

Pi 0.0225

MarkerSeek score 73.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 75
pi: 0.0225
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACGGCCAGCCAAAATGATTA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	58.2	58.1	532-849 bp	1.0	89.7
psbM-petN_p2	ACGGCCAGCCAAAATGATTA	GTATGGGGGAGAAGTGGACT	45.0	55.0	58.2	58.1	531-848 bp	1.0	89.7
psbM-petN_p3	TATGTAAAAACGGCCAGCCA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	57.8	58.1	541-858 bp	1.0	89.2
psbM-petN_p4	TATGTAAAAACGGCCAGCCA	GTATGGGGGAGAAGTGGACT	45.0	55.0	57.8	58.1	540-857 bp	1.0	89.2
psbM-petN_p5	ACGGCCAGCCAAAATGATTA	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.2	58.2	590-907 bp	1.0	89.0