Hotspot

trnD-GUC-psbM

LSC · 15677-16487 · 811 bp

Back to Eriachne

Pi 0.0169

MarkerSeek score 72.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 74
pi: 0.0169
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TAATCATTTTGGCTGGCCGT	50.0	45.0	58.5	58.2	967-1000 bp	1.0	83.7
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	TGGCTGGCCGTTTTTACATA	50.0	45.0	58.5	57.8	958-991 bp	1.0	83.4
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	TTTTGGCTGGCCGTTTTTAC	50.0	45.0	58.5	57.8	961-994 bp	1.0	83.2
trnD-GUC-psbM_p4	TAGATATGCCATACACGCCG	TAATCATTTTGGCTGGCCGT	50.0	45.0	57.4	58.2	1011 bp	0.043478	35.3
trnD-GUC-psbM_p5	TAGATATGCCATACACGCCG	TGGCTGGCCGTTTTTACATA	50.0	45.0	57.4	57.8	1002 bp	0.043478	34.9