Hotspot

trnS-GCU-psbD

LSC · 8045-9069 · 1025 bp

Back to Eriachne

Pi 0.0134

MarkerSeek score 67.90

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 68
pi: 0.0134
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	995-1170 bp	1.0	87.3
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	CGGTCTCTTCGTAACCAGTC	45.0	55.0	58.0	57.7	970-1145 bp	1.0	86.7
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	CTCTTCGTAACCAGTCGTCC	45.0	55.0	58.0	57.7	966-1141 bp	1.0	86.7
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GGTCTCTTCGTAACCAGTCG	45.0	55.0	58.0	57.7	969-1144 bp	1.0	86.7
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	ACGGTCTCTTCGTAACCAGT	45.0	50.0	58.0	58.4	971-1146 bp	1.0	85.6