Hotspot

rps16-trnQ-UUG

LSC · 5640-6706 · 1067 bp

Back to Eriachne

Pi 0.0186

MarkerSeek score 69.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1067
variable_sites: 100
pi: 0.0186
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCTCAAAAGGGCAGCAACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1544-1628 bp	1.0	89.6
rps16-trnQ-UUG_p2	TCTCAAAAGGGCAGCAACAT	TGACAGGCATAACATCCACG	45.0	50.0	58.0	58.0	2071-2155 bp	1.0	88.9
rps16-trnQ-UUG_p3	TCTCAAAAGGGCAGCAACAT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	2120-2204 bp	1.0	88.5
rps16-trnQ-UUG_p4	TCTCAAAAGGGCAGCAACAT	GCTTGCCAAACAAACGCTAA	45.0	45.0	58.0	58.2	2111-2195 bp	1.0	87.7
rps16-trnQ-UUG_p5	TCTCAAAAGGGCAGCAACAT	AAATGGCATAAGCTTCGGGT	45.0	45.0	58.0	57.9	2041-2125 bp	0.913043	83.0