Hotspot

rps15

SSC · 123861-124130 · 270 bp

Back to Eremurus

Pi 0.00709

MarkerSeek score 28.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 270
variable_sites: 7
pi: 0.00709
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TTTCCAATCGTTCTGGTGCA	ACAACGGCAGTCATTTCAGT	45.0	45.0	58.0	58.0	1071-1086 bp	1.0	84.4
rps15_p2	TTTCCAATCGTTCTGGTGCA	CCAACAACGGCAGTCATTTC	45.0	50.0	58.0	57.9	1074-1089 bp	1.0	84.2
rps15_p3	TTTCCAATCGTTCTGGTGCA	CCGAAAGATATATGCCCGCA	45.0	50.0	58.0	57.9	959-973 bp	1.0	84.2
rps15_p4	CCACCGTGTTACATAAGGCA	ACAACGGCAGTCATTTCAGT	50.0	45.0	57.9	58.0	1010-1025 bp	1.0	84.0
rps15_p5	TTTCTTGTAGCCGGTACAGC	ACAACGGCAGTCATTTCAGT	50.0	45.0	57.9	58.0	823-838 bp	0.619048	69.3