Hotspot

rps15-ycf1

SSC · 124131-128848 · 4718 bp

Back to Eremurus

Pi 0.0101

MarkerSeek score 53.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 4718
variable_sites: 205
pi: 0.0101
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGTATGACCATCGAGGAACC	ACATTTGACTTTGGCGAGGA	55.0	45.0	57.9	57.7	229 bp	1.0	80.9
rps15-ycf1_p2	CGTATGACCATCGAGGAACC	TTGACTTTGGCGAGGAATCA	55.0	45.0	57.9	57.4	225 bp	1.0	78.7
rps15-ycf1_p3	CGTATGACCATCGAGGAACC	TGACTTTGGCGAGGAATCAT	55.0	45.0	57.9	57.2	224 bp	1.0	76.9
rps15-ycf1_p4	CGTATGACCATCGAGGAACC	TGACTTTGGCGAGGAATCATT	55.0	42.9	57.9	57.9	224 bp	1.0	74.6
rps15-ycf1_p5	CGTATGACCATCGAGGAACC	TTGACTTTGGCGAGGAATCAT	55.0	42.9	57.9	57.9	225 bp	1.0	74.6