Hotspot

ycf1-ndhF

SSC · 111123-111271 · 149 bp

Back to Eremurus

Pi 0.0563

MarkerSeek score 65.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 149
variable_sites: 28
pi: 0.0563
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	ATGGGGGAAGGGGTCAAATA	50.0	50.0	58.0	58.0	687-907 bp	1.0	88.1
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	TGGGGGAAGGGGTCAAATAT	50.0	50.0	58.0	58.0	686-906 bp	1.0	88.1
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	ATGGGGGAAGGGGTCAAATA	50.0	50.0	58.0	58.0	679-899 bp	1.0	88.1
ycf1-ndhF_p4	AAGAGGGATCCACCGAAGAA	TGGGGGAAGGGGTCAAATAT	50.0	50.0	58.0	58.0	678-898 bp	1.0	88.1
ycf1-ndhF_p5	ACAGGAACAAGAGGGATCCA	TATGGGGGAAGGGGTCAAAT	50.0	50.0	58.0	58.0	703-908 bp	0.952381	85.8