Hotspot

rpl36-rps8

LSC · 80317-80778 · 462 bp

Back to Eremurus

Pi 0.011

MarkerSeek score 57.50

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 27
pi: 0.011
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TCAGTCGGCACTTTTCACAA	AGGCGGAATGGGAATTGTAA	45.0	45.0	58.0	57.2	613-629 bp	1.0	78.0
rpl36-rps8_p2	TCAGTCGGCACTTTTCACAA	TTAGGCGGAATGGGAATTGT	45.0	45.0	58.0	57.2	615-631 bp	1.0	78.0
rpl36-rps8_p3	GGATCAGTCGGCACTTTTCA	AGGCGGAATGGGAATTGTAA	50.0	45.0	58.2	57.2	616-632 bp	1.0	76.7
rpl36-rps8_p4	GGATCAGTCGGCACTTTTCA	TTAGGCGGAATGGGAATTGT	50.0	45.0	58.2	57.2	618-634 bp	1.0	76.7
rpl36-rps8_p5	ATCAGTCGGCACTTTTCACA	AGGCGGAATGGGAATTGTAA	45.0	45.0	57.7	57.2	614-630 bp	1.0	76.2