Hotspot

ycf4-cemA

LSC · 60592-61444 · 853 bp

Back to Eremurus

Pi 0.00901

MarkerSeek score 65.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 35
pi: 0.00901
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCGGAATTGG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	969-1010 bp	1.0	82.3
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	ATGCAAGATAGGGGAGGGAA	45.0	50.0	57.8	57.8	933-974 bp	1.0	82.0
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	AGATGCAAGATAGGGGAGGG	45.0	55.0	57.8	58.3	935-976 bp	1.0	81.6
ycf4-cemA_p4	ACAAAAAGCTGCGGAATTGG	GATGCAAGATAGGGGAGGGA	45.0	55.0	57.8	58.3	934-975 bp	1.0	81.6
ycf4-cemA_p5	TGCGGAATTGGCCTATTTCT	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.6	57.8	960-1001 bp	1.0	80.0