Hotspot

trnF-GAA-ndhJ

LSC · 47988-48701 · 714 bp

Back to Eremurus

Pi 0.00752

MarkerSeek score 55.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 30
pi: 0.00752
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	451-863 bp	1.0	85.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	482-894 bp	1.0	83.5
trnF-GAA-ndhJ_p3	TCGGGATAGCTCAGTTGGTA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.6	57.9	489-901 bp	1.0	82.3
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	454-866 bp	1.0	82.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	450-862 bp	1.0	81.8