Hotspot

psbC-trnS-UGA

LSC · 35004-35142 · 139 bp

Back to Eremurus

Pi 0.0282

MarkerSeek score 60.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 10
pi: 0.0282
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTGTGGGTCATTTGTGGCAT	GGTGGCTGAGTGGTTGATAG	45.0	55.0	58.3	58.0	331-334 bp	1.0	80.6
psbC-trnS-UGA_p2	TTGTGGGTCATTTGTGGCAT	GCTGAGTGGTTGATAGCTCC	45.0	55.0	58.3	58.1	327-330 bp	1.0	80.5
psbC-trnS-UGA_p3	TTGTGGGTCATTTGTGGCAT	GGCTGAGTGGTTGATAGCTC	45.0	55.0	58.3	58.1	328-331 bp	1.0	80.5
psbC-trnS-UGA_p4	TTGTGGGTCATTTGTGGCAT	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.3	58.1	320-323 bp	1.0	80.5
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGTGGCTGAGTGGTTGATAG	45.0	55.0	58.4	58.0	294-297 bp	1.0	80.3