Hotspot

rps3-rpl22

LSC · 79208-79267 · 60 bp

Back to Eremopyrum

Pi 0.0345

MarkerSeek score 75.40

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 2
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTTT	50.0	55.0	57.9	59.6	215-217 bp	1.0	68.5
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	CGAGCTCGAGGACGTAGTTT	50.0	55.0	57.8	59.6	181-183 bp	1.0	67.4
rps3-rpl22_p3	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTT	50.0	57.9	57.9	58.9	215-217 bp	1.0	65.7
rps3-rpl22_p4	CCAAGTCTGAAACCGAGTGG	CGAGCTCGAGGACGTAGTTT	55.0	55.0	58.5	59.6	180-182 bp	1.0	65.4
rps3-rpl22_p5	CAAGTCTGAAACCGAGTGGA	CGAGCTCGAGGACGTAGTTT	50.0	55.0	57.5	59.6	179-181 bp	1.0	65.1