Hotspot

clpP-psbB

LSC · 67540-68049 · 510 bp

Back to Eremopyrum

Pi 0.00206

MarkerSeek score 62.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 510
variable_sites: 1
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GTCGCTTCTTCATCTCCAGG	CCAGCCCAACCAGAAACTAG	55.0	55.0	58.1	58.2	646-670 bp	1.0	81.2
clpP-psbB_p2	GTCGCTTCTTCATCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	562-586 bp	1.0	81.2
clpP-psbB_p3	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.9	58.2	652-676 bp	1.0	80.9
clpP-psbB_p4	TTCATCTCCAGGAATCCGGT	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.1	58.2	638-662 bp	1.0	80.8
clpP-psbB_p5	TCAACCCAAGTCGCTTCTTC	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.1	58.2	655-679 bp	1.0	80.8