Hotspot

psaJ-rpl33

LSC · 63996-64435 · 440 bp

Back to Eremopyrum

Pi 0.0289

MarkerSeek score 73.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 11
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	50.0	55.0	57.3	58.1	583-643 bp	1.0	78.5
psaJ-rpl33_p2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	50.0	50.0	57.3	58.2	560-620 bp	1.0	78.3
psaJ-rpl33_p3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	50.0	50.0	57.3	58.2	572-632 bp	1.0	77.7
psaJ-rpl33_p4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	55.0	55.0	58.9	58.1	582-642 bp	1.0	77.2
psaJ-rpl33_p5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	55.0	50.0	58.9	58.2	559-619 bp	1.0	77.0