Hotspot

trnL-UAA-trnF-GAA

LSC · 47380-47730 · 351 bp

Back to Eremopyrum

Pi 0.0202

MarkerSeek score 61.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 7
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.4	58.1	436-440 bp	1.0	78.4
trnL-UAA-trnF-GAA_p2	GTTGTGAGGGTTCAAGTCCC	CTGCCAGGAACCAGATTTGA	55.0	50.0	58.4	57.8	451-455 bp	1.0	77.1
trnL-UAA-trnF-GAA_p3	GTTGTGAGGGTTCAAGTCCC	TCTGCCAGGAACCAGATTTG	55.0	50.0	58.4	57.8	452-456 bp	1.0	77.1
trnL-UAA-trnF-GAA_p4	GTTGTGAGGGTTCAAGTCCC	TTTTCTCTGCCAGGAACCAG	55.0	50.0	58.4	57.7	457-461 bp	1.0	76.5
trnL-UAA-trnF-GAA_p5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	437-441 bp	1.0	76.5