Hotspot

trnG-UCC-trnM-CAU

LSC · 13593-14722 · 1130 bp

Back to Eremopyrum

Pi 0.00979

MarkerSeek score 64.90

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 11
pi: 0.00979
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1213-1214 bp	1.0	41.9
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	38.1	47.6	55.5	57.5	1273-1274 bp	1.0	41.1
trnG-UCC-trnM-CAU_p3	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1209-1210 bp	1.0	41.1
trnG-UCC-trnM-CAU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	1210-1211 bp	1.0	41.1
trnG-UCC-trnM-CAU_p5	GAACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	1215-1216 bp	1.0	41.1