Hotspot

trnK-UUU-rps16

LSC · 4038-4553 · 516 bp

Back to Eremopyrum

Pi 0.0117
MarkerSeek score 63.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
516
variable_sites
6
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GCTCAACCGACAAGAACTGT 50.0 50.0 58.6 58.4 647-650 bp 1.0 73.5
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG GTGCTCAACCGACAAGAACT 50.0 50.0 58.6 58.4 649-652 bp 1.0 73.5
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TGCTCAACCGACAAGAACTG 50.0 50.0 58.6 58.4 648-651 bp 1.0 73.4
trnK-UUU-rps16_p4 TCGCACTTAAAAGCCGAGTA GCTCAACCGACAAGAACTGT 45.0 50.0 57.3 58.4 645-648 bp 1.0 72.2
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GTGCTCAACCGACAAGAACT 45.0 50.0 57.3 58.4 647-650 bp 1.0 72.2