Hotspot

ycf3

LSC · 43387-45397 · 2011 bp

Back to Eremopogon

Pi 0.0035

MarkerSeek score 52.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2011
variable_sites: 7
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGA	45.0	38.1	57.2	52.5	2077-2093 bp	1.0	40.7
ycf3_p2	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGAA	45.0	36.4	57.2	53.4	2077-2093 bp	1.0	40.7
ycf3_p3	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGA	45.0	36.4	57.2	53.4	2078-2094 bp	1.0	40.7
ycf3_p4	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGAA	45.0	34.8	57.2	54.3	2078-2094 bp	1.0	40.7
ycf3_p5	ATCTTCAACCAAACCACCCT	TCTATATAGAATGGGTTGGGA	45.0	38.1	56.9	52.5	2078-2094 bp	1.0	40.7