Hotspot

rpl32-trnL-UAG

SSC · 107641-108174 · 534 bp

Back to Eremopogon

Pi 0.0094

MarkerSeek score 63.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 534
variable_sites: 5
pi: 0.0094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	630-633 bp	1.0	77.5
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	633-636 bp	1.0	77.1
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	603-606 bp	1.0	76.6
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	596-599 bp	1.0	76.1
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	617-620 bp	1.0	76.1