Hotspot

rpl20-rps12

LSC · 67855-68538 · 684 bp

Back to Eremopogon

Pi 0.00439

MarkerSeek score 61.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 3
pi: 0.00439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	45.0	50.0	58.0	58.0	855-856 bp	1.0	79.9
rpl20-rps12_p2	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.9	58.0	870-871 bp	1.0	79.0
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	TGCAAGACAGCCAATACGAA	45.0	45.0	58.0	57.8	822-823 bp	1.0	78.9
rpl20-rps12_p4	TTTTTGTTCGTCGTCTCCGA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.8	58.0	850-851 bp	1.0	78.4
rpl20-rps12_p5	ACGCATTTTTGTTCGTCGTC	GAACATGCGCTAGGGTGTAT	45.0	50.0	58.0	57.8	757-758 bp	1.0	78.4