Hotspot

petN-rpoB

LSC · 18847-20601 · 1755 bp

Back to Eremopogon

Pi 0.00462

MarkerSeek score 62.10

Primer available yes

Label petN-rpoB

Feature Metadata

label_name: petN-rpoB
feature_type: igs
region: LSC
length_bp: 1755
variable_sites: 8
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-rpoB_p1	AGCCCAAGCGAGACTTACTA	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.2	57.8	1876-1893 bp	1.0	78.7
petN-rpoB_p2	AGCCCAAGCGAGACTTACTA	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.2	57.8	1874-1891 bp	1.0	78.7
petN-rpoB_p3	AGCCCAAGCGAGACTTACTA	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.2	57.8	1877-1894 bp	1.0	78.7
petN-rpoB_p4	AGCCCAAGCGAGACTTACTA	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.2	57.8	1864-1881 bp	1.0	78.4
petN-rpoB_p5	AGCCCAAGCGAGACTTACTA	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.2	58.2	1865-1882 bp	1.0	78.1