Hotspot

trnT-GGU-trnE-UUC

LSC · 15882-16351 · 470 bp

Back to Eremopogon

Pi 0.0527

MarkerSeek score 79.60

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 24
pi: 0.0527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	50.0	50.0	57.4	57.7	526-528 bp	1.0	78.6
trnT-GGU-trnE-UUC_p2	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.4	58.3	547-549 bp	1.0	78.5
trnT-GGU-trnE-UUC_p3	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	50.0	55.0	57.4	57.4	523-525 bp	1.0	76.2
trnT-GGU-trnE-UUC_p4	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	50.0	55.0	57.4	57.4	522-524 bp	1.0	76.2
trnT-GGU-trnE-UUC_p5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	58.1	58.3	547-549 bp	1.0	75.1