Hotspot

ycf1

SSC · 124460-129838 · 5379 bp

Back to Eremophila

Pi 0.0102

MarkerSeek score 57.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 856
pi: 0.0102
unique_haplotype_count: 172
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTTTCCAGTGTAGGGGCG	ACACCGTCCCCCATTTTTAC	50.0	50.0	58.2	58.1	80-2762 bp	0.203125	52.8
ycf1_p2	ATTTTTCCAGTGTAGGGGCG	ATTTAGGCAGAACACCGTCC	50.0	50.0	58.2	57.9	80-2773 bp	0.203125	52.5
ycf1_p3	ATTTTTCCAGTGTAGGGGCG	AGGGTGGAAAGTGAGGAAGA	50.0	50.0	58.2	57.9	80-2717 bp	0.203125	52.4
ycf1_p4	ATTTTTCCAGTGTAGGGGCG	AGGGCCCTCTTATCTCTTCC	50.0	55.0	58.2	57.9	80-1088 bp	0.197917	49.4
ycf1_p5	ATTTTTCCAGTGTAGGGGCG	GGGCCCTCTTATCTCTTCCT	50.0	55.0	58.2	57.9	80-1087 bp	0.197917	49.4