Hotspot

ndhF

SSC · 110900-113131 · 2232 bp

Back to Eremophila

Pi 0.0069

MarkerSeek score 51.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 291
pi: 0.0069
unique_haplotype_count: 132
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATTTAGGCAGAACACCGTCC	TTGCGCTTTGCCAATTCATT	50.0	40.0	57.9	57.8	80-2989 bp	0.796875	81.7
ndhF_p2	ATTTAGGCAGAACACCGTCC	TTTGCGCTTTGCCAATTCAT	50.0	40.0	57.9	57.8	80-2990 bp	0.796875	81.7
ndhF_p3	ACACCGTCCCCCATTTTTAC	ATTTGCGCTTTGCCAATTCA	50.0	40.0	58.1	57.8	80-2980 bp	0.770833	80.8
ndhF_p4	ACACCGTCCCCCATTTTTAC	TTGCGCTTTGCCAATTCATT	50.0	40.0	58.1	57.8	80-2978 bp	0.770833	80.8
ndhF_p5	ACACCGTCCCCCATTTTTAC	TTTGCGCTTTGCCAATTCAT	50.0	40.0	58.1	57.8	80-2979 bp	0.770833	80.8