Hotspot

rpl32-trnL-UAG

SSC · 113849-114725 · 877 bp

Back to Eremophila

Pi 0.0178
MarkerSeek score 68.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
877
variable_sites
270
pi
0.0178
unique_haplotype_count
177
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATGAATTGGCAAAGCGCAAA TTTAGGAGATGACATGCCGC 40.0 50.0 57.8 58.0 80-1268 bp 0.942708 88.0
rpl32-trnL-UAG_p2 TGAATTGGCAAAGCGCAAAT TTTAGGAGATGACATGCCGC 40.0 50.0 57.8 58.0 80-1267 bp 0.942708 88.0
rpl32-trnL-UAG_p3 ATGAATTGGCAAAGCGCAAA CCCCTTTTCGGATGAATCGT 40.0 50.0 57.8 58.0 80-2536 bp 0.052083 51.3
rpl32-trnL-UAG_p4 TGAATTGGCAAAGCGCAAAT CCCCTTTTCGGATGAATCGT 40.0 50.0 57.8 58.0 80-2535 bp 0.052083 51.3
rpl32-trnL-UAG_p5 AATGAATTGGCAAAGCGCAA CCCCTTTTCGGATGAATCGT 40.0 50.0 57.8 58.0 80-2537 bp 0.052083 50.8