Hotspot

atpB-rbcL

LSC · 55811-56589 · 779 bp

Back to Eremophila

Pi 0.0051

MarkerSeek score 65.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 72
pi: 0.0051
unique_haplotype_count: 84
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	80-890 bp	0.994792	87.3
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.1	58.3	80-931 bp	0.989583	86.7
atpB-rbcL_p3	CGGCGGAAAAGCTACATCTA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	80-962 bp	1.0	86.6
atpB-rbcL_p4	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	80-937 bp	1.0	86.3
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	80-891 bp	0.994792	85.2