Hotspot

ndhC-trnV-UAC

LSC · 51611-52793 · 1183 bp

Back to Eremophila

Pi 0.00798

MarkerSeek score 72.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 176
pi: 0.00798
unique_haplotype_count: 135
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	80-1349 bp	1.0	89.8
ndhC-trnV-UAC_p2	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	80-1348 bp	1.0	87.0
ndhC-trnV-UAC_p3	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	80-1370 bp	0.989583	86.7
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	80-1337 bp	1.0	85.9
ndhC-trnV-UAC_p5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	80-1362 bp	1.0	85.9