Hotspot

trnF-GAA-ndhJ

LSC · 49296-49934 · 639 bp

Back to Eremophila

Pi 0.00721

MarkerSeek score 67.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 69
pi: 0.00721
unique_haplotype_count: 76
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	80-818 bp	0.932292	87.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	80-782 bp	0.932292	87.8
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGATAATCATCCGCGCTTGA	55.0	45.0	58.0	57.5	80-858 bp	1.0	86.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	80-806 bp	0.932292	86.9
trnF-GAA-ndhJ_p5	GAATGGTCGGGATAGCTCAG	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.6	58.1	80-826 bp	0.932292	84.3