Hotspot

petN-psbM

LSC · 29669-30652 · 984 bp

Back to Eremophila

Pi 0.00876

MarkerSeek score 68.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 143
pi: 0.00876
unique_haplotype_count: 109
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	80-1123 bp	1.0	80.3
petN-psbM_p2	ACTCGTAGTGTGGGGAAGAA	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	80-1125 bp	0.994792	80.1
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.9	57.5	80-1122 bp	1.0	79.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.2	57.5	80-1177 bp	1.0	79.1
petN-psbM_p5	GTAGTGTGGGGAAGAAGTGG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.5	57.5	80-1121 bp	1.0	76.5