Hotspot

trnC-GCA-petN

LSC · 28737-29578 · 842 bp

Back to Eremophila

Pi 0.0085

MarkerSeek score 64.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 132
pi: 0.0085
unique_haplotype_count: 96
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	80-993 bp	1.0	91.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	80-991 bp	1.0	91.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	80-994 bp	1.0	90.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTAGAGCCCACTTCTTCCC	50.0	55.0	58.1	58.1	80-1003 bp	0.994792	90.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	80-939 bp	0.994792	89.9