Hotspot

atpH-atpI

LSC · 14147-15171 · 1025 bp

Back to Eremophila

Pi 0.00674

MarkerSeek score 64.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 134
pi: 0.00674
unique_haplotype_count: 131
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1002-1306 bp	1.0	86.8
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	917-1221 bp	0.994792	86.8
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	935-1239 bp	0.994792	86.5
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	934-1238 bp	0.994792	85.9
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	929-1233 bp	1.0	85.8