Hotspot

rpl16

LSC · 71442-72598 · 1157 bp

Back to Eremogone

Pi 0.00484

MarkerSeek score 49.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1157
variable_sites: 13
pi: 0.00484
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGGGTAATCGCACCAAAAAC	TGTGAGCCGAAAACTCAACA	50.0	45.0	58.0	58.0	2046-2059 bp	1.0	81.1
rpl16_p2	GTAATCGCACCAAAAACCCG	TGTGAGCCGAAAACTCAACA	50.0	45.0	58.0	58.0	2043-2056 bp	1.0	81.1
rpl16_p3	GTAATCGCACCAAAAACCCG	ACAAACCATTCGCGCAAAAA	50.0	40.0	58.0	58.1	1800-1813 bp	1.0	81.0
rpl16_p4	CGGGTAATCGCACCAAAAAC	ACTTGTCTTTCGCTTTCGGT	50.0	45.0	58.0	58.1	1694-1707 bp	0.8	72.9
rpl16_p5	GTAATCGCACCAAAAACCCG	ACTTGTCTTTCGCTTTCGGT	50.0	45.0	58.0	58.1	1691-1704 bp	0.8	72.9