Hotspot

accD

LSC · 50265-51677 · 1413 bp

Back to Eremogone

Pi 0.00198

MarkerSeek score 63.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1413
variable_sites: 6
pi: 0.00198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAAGCTCT	ACTTTTGGACACAAAGAATTGA	50.0	31.8	58.4	54.7	1439-1469 bp	1.0	42.8
accD_p2	AATAGGGGGCAAGAAAGCTC	ACTTTTGGACACAAAGAATTGA	50.0	31.8	57.6	54.7	1440-1470 bp	1.0	42.8
accD_p3	ATAGGGGGCAAGAAAGCTCT	AACTTTTGGACACAAAGAATTGA	50.0	30.4	58.4	55.4	1440-1470 bp	1.0	42.8
accD_p4	AATAGGGGGCAAGAAAGCTC	AACTTTTGGACACAAAGAATTGA	50.0	30.4	57.6	55.4	1441-1471 bp	1.0	42.8
accD_p5	ATAGGGGGCAAGAAAGCTCT	ACTTTTGGACACAAAGAATTGAT	50.0	30.4	58.4	55.2	1439-1469 bp	1.0	42.8