Hotspot

ndhF-rpl32

SSC · 98062-98469 · 408 bp

Back to Eremogone

Pi 0.0221

MarkerSeek score 61.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 408
variable_sites: 14
pi: 0.0221
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGAAGGGTATGATCCATGCA	ACGAATACGCTTTTTCGAAA	45.0	35.0	56.3	54.3	496-539 bp	1.0	43.7
ndhF-rpl32_p2	TTGGAAGTGGAATGAAGGGT	ACGAATACGCTTTTTCGAAA	45.0	35.0	56.6	54.3	508-551 bp	1.0	43.6
ndhF-rpl32_p3	CCCACTCCGATTAACAAAGGA	ACGAATACGCTTTTTCGAAA	47.6	35.0	57.7	54.3	530-573 bp	1.0	43.5
ndhF-rpl32_p4	TCCCACTCCGATTAACAAAGG	ACGAATACGCTTTTTCGAAA	47.6	35.0	57.7	54.3	531-574 bp	1.0	43.5
ndhF-rpl32_p5	CCCACTCCGATTAACAAAGGA	ACGAATACGCTTTTTCGAAAT	47.6	33.3	57.7	54.9	530-573 bp	1.0	43.5