Hotspot

trnF-GAA-ndhJ

LSC · 41669-42251 · 583 bp

Back to Eremogone

Pi 0.0134
MarkerSeek score 62.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
583
variable_sites
15
pi
0.0134
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA CCCGAAAGTTGGGTAGGATG 55.0 55.0 58.0 58.0 712-731 bp 1.0 83.7
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA CCGAAAGTTGGGTAGGATGG 55.0 55.0 58.0 58.0 711-730 bp 1.0 83.7
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCCGAAAGTTGGGTAGGATG 50.0 55.0 58.1 58.0 676-695 bp 1.0 83.5
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CCGAAAGTTGGGTAGGATGG 50.0 55.0 58.1 58.0 675-694 bp 1.0 83.5
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA ATCCGCGCCTAAAACGTATT 55.0 45.0 58.0 58.1 738-757 bp 1.0 83.4